The use of AI in medical practice offers a number of significant and visible advantages. The problem of the presented research is relevant due to the growing integration of technologies into healthcare. The purpose of this study is to analyze the ethical and cultural challenges associated with integration of artificial intelligence (AI) in medical practice in various cultural contexts. The main objective of the study is to identify specific problems and suggest possible solutions to ensure effective and justifiable use of AI. To achieve this goal, a literary review, a case study, an expert interview, and a questionnaire were used. The main areas of ethical and cultural challenges include the issues of confidentiality and data protection; culturally specific attitudes towards automation of medical decision-making; the impact of algorithm bias on the diagnosis and treatment of various ethnic groups; ethical dilemmas related to access and fairness in the use of medical AI systems. The study highlights the need to develop ethical standards for the use of AI in medicine that will take into account cultural differences.