In this article, the issues of digital literacy that arise during communication of medical and pharmaceutical workers have been reviewed. The right for digital literacy, access to education that improves comprehension and develops digital skills while using medical information systems relates to one of the issues. The goal of the research is to assess digital literacy of healthcare professionals, which is essential for professional interaction during clinical research. Applicable laws and scientific publications regulating healthcare digitalization were used as instructional material. Circulation of medicinal preparations used in clinical research was examined through summary reports of medical companies in 2022–2024. Data from registered databases served as a basis for sociological research to determine digital literacy of professionals. According to the survey results, over 90.0% of respondents have computer-assisted workplaces, about 50.0% of them assessed their level of digital competence as average, and 67.4% need to improve their digital literacy. Optimal organizational solutions allow to improve digital literacy of all healthcare professionals producing a positive effect on ethics of professional interaction.